An immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mapping

Simple item page
dc.contributor.authorVelmurugan, Janaki
dc.contributor.authorMilbourne, Dan
dc.contributor.authorConnolly, Vincent
dc.contributor.authorHeslop-Harrison, J. S.
dc.contributor.authorAnhalt, Ulrike C. M.
dc.contributor.authorLynch, M. B.
dc.contributor.authorBarth, Susanne
dc.contributor.funderTeagasc
dc.date.accessioned2018-09-20T15:53:44Z
dc.date.available2018-09-20T15:53:44Z
dc.date.issued2018
dc.description.abstractTo address the lack of a truly portable, universal reference mapping population for perennial ryegrass, we have been developing a recombinant inbred line (RIL) mapping population of perennial ryegrass derived via single seed descent from a well-characterized F2 mapping population based on genetically distinct inbred parents in which the natural self-incompatibility (SI) system of perennial ryegrass has been overcome. We examined whether it is possible to create a genotyping by sequencing (GBS) based genetic linkage map in a small population of the F6 generation of this population. We used 41 F6 genotypes for GBS with PstI/MspI-based libraries. We successfully developed a genetic linkage map comprising 6074 SNP markers, placing a further 22080 presence and absence variation (PAV) markers on the map. We examined the resulting genetic map for general and RIL specific features. Overall segregation distortion levels were similar to those experienced in the F2 generation, but segregation distortion was reduced on linkage group 6 and increased on linkage group 7. Residual heterozygosity in the F6 generation was observed at a level of 5.4%. There was a high proportion of chromosomes (30%) exhibiting the intact haplotype of the original inbred parents of the F1 genotype from which the population is derived, pointing to a tendency for chromosomes to assort without recombining. This could affect the applicability of these lines and might make them more suitable for situations where repressed recombination is an advantage. Inter-and intra-chromosomal linkage disequilibrium (LD) analysis suggested that the map order was robust. We conclude that this RIL population, and subsequent F7 and F8 generations will be useful for genetic analysis and phenotyping of agronomic and biological important traits in perennial ryegrass.en
dc.description.sponsorshipTeagasc (Walsh Ph.D. Fellowships)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid9
dc.identifier.citationVelmurugan, J., Milbourne, D., Connolly, V., Heslop-Harrison, J. S., Anhalt, U. C. M., Lynch, M. B. and Barth, S. (2018) 'An immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mapping', Frontiers in Plant Science, 9, 717 (13pp). doi: 10.3389/fpls.2018.00717en
dc.identifier.doi10.3389/fpls.2018.00717
dc.identifier.endpage13
dc.identifier.issn1664-462X
dc.identifier.journaltitleFrontiers in Plant Scienceen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6840
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fpls.2018.00717/full
dc.rights© 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPerennial ryegrassen
dc.subjectLolium perenneen
dc.subjectRecombinant inbred lines (RIL)en
dc.subjectGenotyping by sequencingen
dc.subjectMapping populationen
dc.subjectPhenotypingen
dc.titleAn immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mappingen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 5 of 5
Thumbnail Image
Name:
An Immortalized Genetic Mapping.pdf
Size:
2.94 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Thumbnail Image
Name:
An Immortalized Genetic Mapping-1.docx
Size:
14.7 KB
Format:
Microsoft Word XML
Description:
Supplemental File 1
Download
Thumbnail Image
Name:
An Immortalized Genetic Mapping-2.docx
Size:
956.76 KB
Format:
Microsoft Word XML
Description:
Supplemental File 2
Download
Thumbnail Image
Name:
An Immortalized Genetic Mapping-3.xlsx
Size:
32.92 KB
Format:
Microsoft Excel XML
Description:
Supplemental File 3
Download
Thumbnail Image
Name:
An Immortalized Genetic Mapping-4.docx
Size:
17.42 KB
Format:
Microsoft Word XML
Description:
Supplemental File 4
Download
Collections
APC Microbiome Ireland - Journal Articles