An immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mapping

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dc.contributor.author Velmurugan, Janaki
dc.contributor.author Milbourne, Dan
dc.contributor.author Connolly, Vincent
dc.contributor.author Heslop-Harrison, J. S.
dc.contributor.author Anhalt, Ulrike C. M.
dc.contributor.author Lynch, M. B.
dc.contributor.author Barth, Susanne
dc.date.accessioned 2018-09-20T15:53:44Z
dc.date.available 2018-09-20T15:53:44Z
dc.date.issued 2018
dc.identifier.citation Velmurugan, J., Milbourne, D., Connolly, V., Heslop-Harrison, J. S., Anhalt, U. C. M., Lynch, M. B. and Barth, S. (2018) 'An immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mapping', Frontiers in Plant Science, 9, 717 (13pp). doi: 10.3389/fpls.2018.00717 en
dc.identifier.volume 9
dc.identifier.startpage 1
dc.identifier.endpage 13
dc.identifier.issn 1664-462X
dc.identifier.uri http://hdl.handle.net/10468/6840
dc.identifier.doi 10.3389/fpls.2018.00717
dc.description.abstract To address the lack of a truly portable, universal reference mapping population for perennial ryegrass, we have been developing a recombinant inbred line (RIL) mapping population of perennial ryegrass derived via single seed descent from a well-characterized F2 mapping population based on genetically distinct inbred parents in which the natural self-incompatibility (SI) system of perennial ryegrass has been overcome. We examined whether it is possible to create a genotyping by sequencing (GBS) based genetic linkage map in a small population of the F6 generation of this population. We used 41 F6 genotypes for GBS with PstI/MspI-based libraries. We successfully developed a genetic linkage map comprising 6074 SNP markers, placing a further 22080 presence and absence variation (PAV) markers on the map. We examined the resulting genetic map for general and RIL specific features. Overall segregation distortion levels were similar to those experienced in the F2 generation, but segregation distortion was reduced on linkage group 6 and increased on linkage group 7. Residual heterozygosity in the F6 generation was observed at a level of 5.4%. There was a high proportion of chromosomes (30%) exhibiting the intact haplotype of the original inbred parents of the F1 genotype from which the population is derived, pointing to a tendency for chromosomes to assort without recombining. This could affect the applicability of these lines and might make them more suitable for situations where repressed recombination is an advantage. Inter-and intra-chromosomal linkage disequilibrium (LD) analysis suggested that the map order was robust. We conclude that this RIL population, and subsequent F7 and F8 generations will be useful for genetic analysis and phenotyping of agronomic and biological important traits in perennial ryegrass. en
dc.description.sponsorship Teagasc (Walsh Ph.D. Fellowships) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media S.A. en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fpls.2018.00717/full
dc.rights © 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Perennial ryegrass en
dc.subject Lolium perenne en
dc.subject Recombinant inbred lines (RIL) en
dc.subject Genotyping by sequencing en
dc.subject Mapping population en
dc.subject Phenotyping en
dc.title An immortalized genetic mapping population for perennial ryegrass: a resource for phenotyping and complex trait mapping en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Janaki Velmurugan, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 E-mail: janaki.velmurugan@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Teagasc
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Plant Science en
dc.internal.IRISemailaddress janaki.velmurugan@ucc.ie en
dc.identifier.articleid 9


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© 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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